Nicolas Buchon
Assistant Professor

Nicolas Buchon

Phone

607-255-7828

Address

Department of Entomology
Cornell University
5124 Comstock Hall
Ithaca, NY  14853

Email

Web Sites

Lab Web Site
Department Profile

Background

Nicolas Buchon is an Assistant Professor in the Department of Entomology.  He received a B.S. degree in 2001 and a M.S. degree in 2003 in Cellular and Molecular Biology from the Universite d’Auvergne Clermont I, France.  In 2006, he was awarded a Ph.D. degree in Physiology and Molecular Genetics.

Research Description

My laboratory uses the genetic model Drosophila melanogaster to study the host response to indigenous and pathogenic microbes using genomics, functional genetics and systems-level approaches. We focus on the integration of immune, growth and repair mechanisms in the gut.

We are interested in understanding Host-Microbe relationships and the genetic network that governs the host response to microbes. Our goal is to integrate the immune response per se with mechanisms employed by the host to repair the infectious damage in an integrative model. The main focus of our work is to characterize the host mechanisms that control intestinal homeostasis in response to infectious and resident microbes. In addition, we want to understand how microbes can alter intestinal homeostasis and give rise to pathologies of gut origin such as intestinal immune disorders or cancers. Finally we aim to characterize the spatial complexity of the gastro-intestinal system, and we study how gut regions are established and maintained and how regional variations in intestinal physiology are relevant to health and disease. We emphasize coupling between genomics, functional genetics, cell biology and systems level approaches (computation and theory) and use the fruit fly as our main model system.

Publications

Buchon N, Broderick NA, Chakrabarti S, Lemaitre B. 2009. Invasive and indigenous microbiota impact intestinal stem cell activity through multiple pathways. Genes Dev. 23(19): 2333-44.

Buchon N, Poidevin M, Kwon HM, Guillou A, Sottas V, Lee BL, Lemaitre B. 2009. A single modular serine protease integrates signals from pattern-recognition receptors upstream of the Drosophila Toll pathway. Proc. Natl. Acad. Sci. USA 106(30): 12442-7.

Buchon N, Broderick NA, Poidevin M, Pradervand S, Lemaitre B. 2009. Drosophila intestinal response to bacterial infection: activation of host defense and stem cell proliferation. Cell Host Microbe 5(2): 200-11.

Lhocine N, Ribeiro PS, Buchon N, Wepf A, Wilson R, Tenev T, Lemaitre B, Gstaiger M, Meier P, Leulier F. 2008. PIMS modulates immune tolerance by negatively regulating Drosophila innate immune signaling. Cell Host Microbe4(2): 147-58. Selected by Faculty of 1000 with mention “Must read”. [pdf]

*Buchon N, *Broderick NA, Kuraishi T, Lemaitre B. 2010. Drosophila EGFR coordinates stem cell proliferation and gut remodeling following infection. BMC Biology 8:152.

Kuraishi T, Binggeli O, Opota O, Buchon N, Lemaitre B. 2011. Genetic evidence for a protective role of the peritrophic matrix against intestinal bacterial infection in Drosophila melanogaster. Proc Natl Acad Sci USA early edition, pp. 1-6.

Chakrabarti S, Liehl P, *Buchon N and *Lemaitre B. 2012. Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. Cell Host Microbe 12(1): 60-70.

Osman D, Buchon N, Chakrabarti S, Huang YT, Su WC, Poidevin M, Tsai YC, Lemaitre B. 2012. Autocrine and paracrine unpaired signalling regulate intestinal stem cell maintenance and division. J. Cell Science 125: 5944-5949.

Buchon N, Osman D, David FPA, Fang HY, Boquete JP, Deplancke B, Lemaitre B. 2013. Morphological and molecular characterization of adult midgut compartmentalization in Drosophila. Cell Reports 3: 1725-1738.