Shu-Bing Qian
Assistant Professor
Haiyun Yu

Phone

607-254-3397

Address

Division of Nutrition
301 Biotechnology Building
Cornell University
Ithaca, NY 14853

Email

Web Sites

Department Profile

Lab Website

Background

Shu-Bing Qian received a M.Sc. degree in 1997 and a Ph.D. degree in Biochemistry in 2000 from Shanghai Jiaotong University Medical School (formerly Shanghai Second Medical University). He was a Postdoctoral Fellow at the National Institutes of Health, Bethesda, MD from 2000-2004 and at the University of North Carolina-Chapel Hill from 2004-2006. He became an Assistant Professor in the Division of Nutritional Sciences at Cornell University in July 2008.

Research Description

How is mRNA translation controlled by nutrient signaling? How does protein folding and degradation occur during protein synthesis? How do cells get rid of misfolded proteins? These are a few of the problems we would like to understand. Elucidation of the molecular mechanisms underlying protein quality and quantity control will ultimately define new therapeutic strategies to human diseases such as cancer, diabetes, and neurodegenerative disorders.

Specifically, we use biochemistry, cell biological and genetic approaches to study translational control of gene expression and protein triage (folding, degradation, and aggregation) using mammalian system. We established multi-dimensional ribosomal profiling analysis to monitor mRNA translation at a sub-codon resolution, which allows us to investigate ribosome dynamics as well as co-translational events. In addition, we are applying genome-wide high-content RNAi screen to dissect protein triage decision. By focusing on chaperone network and ubiquitin ligases, we are dedicated to identify novel regulators of protein quality control.

Selected Publications

Liu, B., Han, Y., and Qian, S.B. (2012) Co-translational response to proteotoxic stress by early ribosome pausing (in revision).
Lee, S., Liu, B., Lee, S., Huang, S.X., Shen, B., and Qian, S.B. (2012) Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution. Proc. Natl. Acad. Sci. USA 109, E2424-32.

Han, Y., David, A., Liu, B., Magadán, J.G., Bennink, J.R., Yewdell, J.W., and Qian, S.B. (2012) Monitoring co-translational protein folding in mammalian cells at codon resolution. Proc. Natl. Acad. Sci. USA 109, 12467-12472.

Park, W.J., Kothapalli, K.S., Reardon, H.T., Lawrence, P., Qian, S.B., and Brenna, J.T. (2012) A novel FADS1 isoform potentiates FADS2-mediated production of eicosanoid precursor fatty acids. J. Lipid Res. 53, 1502-1512

Liu, B. and Qian, S.B. (2011) Translational regulation in nutrigenomics. Adv. Nutr. 2, 511-519.

Zhang, X. and Qian, S.B. (2011) Chaperone-mediated hierarchical control in targeting misfolded proteins to aggresome. Mol. Biol. Cell 22, 3277-3288.

Conn, C.S. and Qian, S.B. (2011) Invited review: Nutrient signaling in protein homeostasis. Cell Cycle 10, 1940-1947

Sun, J., Conn, C.S., Han, Y., Yeung, V., and Qian, S.B. (2011) PI3K-mTORC1 attenuates stress response by inhibiting cap-independent Hsp70 mRNA translation. J. Biol. Chem. 286, 6791-6800.

Qian, S.B., Zhang, X., Sun, J., Bennink, J.R., Yewdell, J.W., and Patterson, C. (2010) mTORC1 links protein quality and quantity control by sensing chaperone availability. J. Biol. Chem. 285, 27385-27395.

Qian, S.B., Waldren, L., Choudhary, N., Klevit, R.E., Chazin, W.J., and Patterson, C. (2009) Engineering a ubiquitin ligase reveals conformational flexibility required for ubiquitin transfer. J. Biol. Chem. 284, 26797-26802.